Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces
Identifieur interne : 002C37 ( Main/Exploration ); précédent : 002C36; suivant : 002C38Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces
Auteurs : Asya Levina [Russie] ; Inna Pyshnaya [Russie] ; Marina Repkova [Russie] ; Vera Rar [Russie] ; Valentina Zarytova [Russie]Source :
- Biotechnology Journal [ 1860-6768 ] ; 2007-07.
English descriptors
- Teeft :
- Babesia, Biotechnol, Biotin label, Chips, Chromogenic substrates, Derivative, Epoxy glass slides, Fragment, Glass slides, Gmbh, Hybridization, Immobilization, Kgaa, Lavsan, Ligation, Ligation product, Nucleic, Nucleic acids, Nucleosides nucleotides, Nucleotide, Nylon, Nylon membranes, Oligonucleotide, Oligonucleotides, Positive control, Potassium phosphate buffer, Room temperature, Rrna gene, Seite, Short oligonucleotides, Solid support, Solid surfaces, Surface capacity, Tandem, Unmodified, Unmodified glass slides, Verlag, Verlag gmbh, Weinheim, Weinheim seite.
Abstract
Various materials, such as glass, plastic, metals, etc., are utilized for preparing DNA chips. In each particular case special approaches are used for immobilization of different oligonucleotide derivatives on the solid supports. We describe a general technique for DNA chips preparation on various unmodified surfaces using one type of oligonucleotide derivative, polylysine‐oligonucleotide conjugates (PL‐oligo). A long polyamine spacer in the PL‐oligo conjugates provides a durable irreversible non‐covalent immobilization onto a variety of solid supports and enough distance between oligonucleotides and the surface. The resulting DNA chips were shown to be useful for the detection of PCR DNA fragments and to be sensitive to single nucleotide discrepancies. They represent a promising instrument for revealing genetic diseases, genotyping viruses and bacteria, and for displaying their drug‐resistant strains.
Url:
DOI: 10.1002/biot.200700027
Affiliations:
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wicri:rule="url">Russie</country></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biotechnology Journal</title><title level="j" type="sub">Biotech in Russia</title><title level="j" type="alt">BIOTECHNOLOGY JOURNAL</title><idno type="ISSN">1860-6768</idno><idno type="eISSN">1860-7314</idno><imprint><biblScope unit="vol">2</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="879">879</biblScope><biblScope unit="page" to="885">885</biblScope><biblScope unit="page-count">7</biblScope><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Weinheim</pubPlace><date type="published" when="2007-07">2007-07</date></imprint><idno type="ISSN">1860-6768</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1860-6768</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Babesia</term><term>Biotechnol</term><term>Biotin label</term><term>Chips</term><term>Chromogenic substrates</term><term>Derivative</term><term>Epoxy glass slides</term><term>Fragment</term><term>Glass slides</term><term>Gmbh</term><term>Hybridization</term><term>Immobilization</term><term>Kgaa</term><term>Lavsan</term><term>Ligation</term><term>Ligation product</term><term>Nucleic</term><term>Nucleic acids</term><term>Nucleosides nucleotides</term><term>Nucleotide</term><term>Nylon</term><term>Nylon membranes</term><term>Oligonucleotide</term><term>Oligonucleotides</term><term>Positive control</term><term>Potassium phosphate buffer</term><term>Room temperature</term><term>Rrna gene</term><term>Seite</term><term>Short oligonucleotides</term><term>Solid support</term><term>Solid surfaces</term><term>Surface capacity</term><term>Tandem</term><term>Unmodified</term><term>Unmodified glass slides</term><term>Verlag</term><term>Verlag gmbh</term><term>Weinheim</term><term>Weinheim seite</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Various materials, such as glass, plastic, metals, etc., are utilized for preparing DNA chips. In each particular case special approaches are used for immobilization of different oligonucleotide derivatives on the solid supports. We describe a general technique for DNA chips preparation on various unmodified surfaces using one type of oligonucleotide derivative, polylysine‐oligonucleotide conjugates (PL‐oligo). A long polyamine spacer in the PL‐oligo conjugates provides a durable irreversible non‐covalent immobilization onto a variety of solid supports and enough distance between oligonucleotides and the surface. The resulting DNA chips were shown to be useful for the detection of PCR DNA fragments and to be sensitive to single nucleotide discrepancies. They represent a promising instrument for revealing genetic diseases, genotyping viruses and bacteria, and for displaying their drug‐resistant strains.</div></front></TEI><affiliations><list><country><li>Russie</li></country></list><tree><country name="Russie"><noRegion><name sortKey="Levina, Asya" sort="Levina, Asya" uniqKey="Levina A" first="Asya" last="Levina">Asya Levina</name></noRegion><name sortKey="Pyshnaya, Inna" sort="Pyshnaya, Inna" uniqKey="Pyshnaya I" first="Inna" last="Pyshnaya">Inna Pyshnaya</name><name sortKey="Rar, Vera" sort="Rar, Vera" uniqKey="Rar V" first="Vera" last="Rar">Vera Rar</name><name sortKey="Repkova, Marina" sort="Repkova, Marina" uniqKey="Repkova M" first="Marina" last="Repkova">Marina Repkova</name><name sortKey="Zarytova, Valentina" sort="Zarytova, Valentina" uniqKey="Zarytova V" first="Valentina" last="Zarytova">Valentina Zarytova</name><name sortKey="Zarytova, Valentina" sort="Zarytova, Valentina" uniqKey="Zarytova V" first="Valentina" last="Zarytova">Valentina Zarytova</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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